Measurement of myo-inositol turnover in phosphatidylinositol: description of a model and mass spectrometric method for cultured cortical neurons.

Abstract

Rates of myo-inositol (Ins) incorporation and turnover in phosphatidylinositol (PtdIns) were determined in cultured mouse cortical neurons. Cells were incubated with deuterium-labeled myo-inositol (Ins) in culture medium free of unlabeled Ins. The time-dependent changes in the specific activity of cytosolic Ins and membrane PtdIns were measured by mass… (More)

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@article{Chikhale2001MeasurementOM, title={Measurement of myo-inositol turnover in phosphatidylinositol: description of a model and mass spectrometric method for cultured cortical neurons.}, author={Elsbeth Chikhale and Andrea Balbo and Zygmunt Galdzicki and Stanley I. Rapoport and H. Umesha Shetty}, journal={Biochemistry}, year={2001}, volume={40 37}, pages={11114-20} }