Measurement of multisite oxidation kinetics reveals an active site conformational change in Spo0F as a result of protein oxidation.


When most proteins undergo oxidative damage, they yield a variety of products containing oxidative damage at a large number of sites, most of which are modified substoichiometrically. The resulting complex mixture of products is not amenable to high-resolution structural analyses. The previous methods of structural analysis have relied upon either very… (More)