Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay.

@article{Beatty1987MeasurementOM,
  title={Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay.},
  author={John David Beatty and Barbara G. Beatty and William G. Vlahos},
  journal={Journal of immunological methods},
  year={1987},
  volume={100 1-2},
  pages={
          173-9
        }
}
Enzyme-linked immunoadsorbent assay (EIA) has widespread use for the measurement of antibody concentration. The affinity constant (Kaff) of the antibody has an effect upon the quantification by EIA. It is thus important to be able to measure Kaff by solid-phase EIA. Based upon the Law of Mass Action and using serial dilutions of both antigens (coating the plate) and antibody, Kaff has been measured by EIA. A microtiter plate was coated with antigen (Ag) and then incubated with monoclonal… CONTINUE READING
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