Measurement of membrane potentials (ψ) of erythrocytes and white adipocytes by the accumulation of triphenylmethylphosphonium cation

@article{Cheng2005MeasurementOM,
  title={Measurement of membrane potentials ($\psi$) of erythrocytes and white adipocytes by the accumulation of triphenylmethylphosphonium cation},
  author={Kang Cheng and Howard C. Haspel and Mary Lou Vallano and Babatunde Osotimehin and Martin Sonenberg},
  journal={The Journal of Membrane Biology},
  year={2005},
  volume={56},
  pages={191-201}
}
SummaryThe accumulation of the lipophilic cation, triphenylmethylphosphonium, has been employed to determine the resting membrane potential in human erythrocytes, turkey erythrocytes, and rat white adipocytes. The triphenylmethylphosphonium cation equilibrates rapidly in human erythrocytes in the presence of low concentrations of the hydrophobic anion, tetraphenylborate. Tetraphenylborate does not accelerate the uptake of triphenylmethylphosphonium ion by adipocytes. The cell associatedvs… 
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23 Citations
Background Citations
6
Methods Citations
2

23 Citations

Citation Type

Citation Type

Triphenylmethylphosphonium cation distribution as a measure of hormone-induced alterations in white adipocyte membrane potential
TLDR
It is suggested that intracellular free fatty acid accumulation in response to lipolytic agents causes dissipation of the mitochondrial membrane potential and efflux of [3H]TPMP+ from the organelle and cell.
Permeability change in transformed mouse fibroblasts caused by ionophores, and its relationship to membrane permeabilization by exogenous ATP
TLDR
The effects exerted by electrogenic ionophores on the plasma membrane were found to be similar to those induced by exogenous ATP, whereas ATP effects are specific for transformed cells, and it is postulated that ATP actsvia specific sites on the surface of transformed cells.
Erythrocyte plasma membrane potential: past and current methods for its measurement
TLDR
This article summarizes the methodologies used during the past decades to determine Δψ in red blood cells, from seminal microelectrodes, through the use of nuclear magnetic resonance or lipophilic radioactive ions to quantify intra and extracellular ions, to continuously renewed fluorescent potentiometric dyes.
Transport of hydrophobic ions in erythrocyte membrane: I. Zero membrane potential properties
TLDR
TPA flux is stimulated by substoichiometric amounts of tetraphenylboron and other organic anions, suggesting an ion-pairing mechanism, and no evidence for facilitated transport could be found.
Cation channels, cell volume and the death of an erythrocyte
TLDR
Beyond their role in the limitation of erythrocyte survival, cation channels may contribute to the triggering of apoptosis in nucleated cells exposed to osmotic shock and/or oxidative stress.
Membrane potential and ion partitioning in an erythrocyte using the Poisson-Boltzmann equation.
TLDR
To investigate how much the dispersion forces contribute to active ion pumps in an erythrocyte (red blood cell) as well as the correction of chemical potential reference states between intracellular and extracellular environments, the modified Poisson-Boltzmann equation is analyzed.
TRPC6 Contributes to the Ca2+ Leak of Human Erythrocytes
TLDR
Human and mouse erythrocytes express TRPC6 cation channels which participate in cation leak and Ca2+-induced suicidal death.
Isoosmotic shrinkage by self-stimulated outward Na-K-Cl cotransport in quail erythrocytes
TLDR
Isoosmotic hypokalaemia drives a rapid shrinkage of quail erythrocytes, due to auto-catalytic net outward cotransport stimulation.
On the physiological and cellular homeostasis of ascorbate
TLDR
The proposed approach provides, for the first time, a mechanistic account of processes leading to ascorbate physiological and cellular distribution, which helps to explain numerous experimental and clinical observations.
Patch-clamp analysis of the "new permeability pathways" in malaria-infected erythrocytes.
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