Measurement of membrane phospholipid asymmetry in normal and sickle-cell erythrocytes by means of annexin V binding.

@article{Tait1994MeasurementOM,
  title={Measurement of membrane phospholipid asymmetry in normal and sickle-cell erythrocytes by means of annexin V binding.},
  author={Jonathan F. Tait and Donald F Gibson},
  journal={The Journal of laboratory and clinical medicine},
  year={1994},
  volume={123 5},
  pages={
          741-8
        }
}
We investigated the use of annexin V (placental anticoagulant protein I), a calcium-dependent protein that binds tightly to phosphatidylserine-containing membranes, as a means to measure membrane phospholipid asymmetry in human erythrocytes. Iodine 125-labeled annexin V bound to erythrocytes in a specific, reversible, calcium-dependent reaction (dissociation constant = 25 +/- 4 nmol/L at 37 degrees C and 2.5 mmol/L calcium). Lysed erythrocytes contained 1.2 x 10(6) binding sites for annexin V… CONTINUE READING
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