Measurement of intracellular free zinc in living cortical neurons: routes of entry.

  title={Measurement of intracellular free zinc in living cortical neurons: routes of entry.},
  author={Stefano L Sensi and Lorella Maria Teresa Canzoniero and Shan Ping Yu and Howard S. Ying and Jae Young Koh and Geoffrey A. Kerchner and Dennis W. Choi},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  volume={17 24},
We used the ratioable fluorescent dye mag-fura-5 to measure intracellular free Zn2+ ([Zn2+]i) in cultured neocortical neurons exposed to neurotoxic concentrations of Zn2+ in concert with depolarization or glutamate receptor activation and identified four routes of Zn2+ entry. Neurons exposed to extracellular Zn2+ plus high K+ responded with a peak cell body signal corresponding to a [Zn2+]i of 35-45 nM. This increase in [Zn2+]i was attenuated by concurrent addition of Gd3+, verapamil, omega… CONTINUE READING
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