Measurement of intracellular free calcium in monkey kidney cells with aequorin.

@article{Borle1982MeasurementOI,
  title={Measurement of intracellular free calcium in monkey kidney cells with aequorin.},
  author={Andr{\'e} Bernard Borle and Kenneth W. Snowdowne},
  journal={Science},
  year={1982},
  volume={217 4556},
  pages={252-4}
}
A method has been developed for the measurement of intracellular free calcium in mammalian cells. The calcium-sensitive photoprotein aequorin can be incorporated into isolated cells by hypo-osmotic treatment without altering the cell viability, permeability, or metabolism. Intracellular calcium activity (Cai2+) was monitored in a perfusion system. In monkey kidney cells (LLC-MK2), Cai2+ is approximately 57 nanomoles per liter. Changes in Cai2+ with time can also be followed: exposure of the… CONTINUE READING

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