Measurement of intracellular calcium levels by the fluorescent Ca(2+) indicator Calcium-Green.

@article{Silei2000MeasurementOI,
  title={Measurement of intracellular calcium levels by the fluorescent Ca(2+) indicator Calcium-Green.},
  author={Valerio Silei and Cinzia Fabrizi and Giulia Venturini and F. Tagliavini and Mario Salmona and Orso Bugiani and Giuliana Lauro},
  journal={Brain research. Brain research protocols},
  year={2000},
  volume={5 2},
  pages={
          132-4
        }
}
The fluorescent calcium-sensitive indicators, such as the Calcium Green-1, allow one to detect small calcium transients at low indicator concentrations. The protocol reported here is a rapid and sensitive method that facilitates the measurement of intracellular free-calcium in cell suspensions. Using this assay, we were able to detect and quantify the variations in intracellular calcium concentration during microglial cell activation induced by the fragment peptides beta25-35 and PrP106-126.