Measurement of interferon from in vitro stimulated lymphocytes by bioassay and monoclonal antibody-based immunoassay.

@article{Scott1985MeasurementOI,
  title={Measurement of interferon from in vitro stimulated lymphocytes by bioassay and monoclonal antibody-based immunoassay.},
  author={Geoffrey M. Scott and Joseph A. Robinson and David S. Secher and C M Ashburner and S R Abbott},
  journal={The Journal of general virology},
  year={1985},
  volume={66 ( Pt 7)},
  pages={1621-5}
}
Peripheral blood mononuclear cells (PBMC) derived from healthy individuals were stimulated with u.v.-inactivated Newcastle disease virus and the cell supernatants were assayed for both antiviral activity and alpha interferon (IFN-alpha) immunoreactivity. IFN-alpha concentrations determined by two immunoradiometric assays (IRMAs) based on monoclonal… CONTINUE READING