Measurement of insulin-like growth factor-II in human plasma using a specific monoclonal antibody-based two-site immunoradiometric assay.

@article{Crosby1993MeasurementOI,
  title={Measurement of insulin-like growth factor-II in human plasma using a specific monoclonal antibody-based two-site immunoradiometric assay.},
  author={Steven R Crosby and Catherine Anderton and Melissa Westwood and Jeff M. P. Holly and S C Cwyfan Hughes and Matthew Gibson and Catherine Anne Morrison and Robert John Young and Anne R White},
  journal={The Journal of endocrinology},
  year={1993},
  volume={137 1},
  pages={141-50}
}
An immunoradiometric assay (IRMA) for the measurement of insulin-like growth factor-II (IGF-II) in human plasma has been developed, optimized and evaluated clinically in normal subjects and patients with disorders of the GH/IGF-I axis. Six monoclonal antibodies (MAbs) to recombinant human IGF-II (rhIGF-II) were produced, all of which had low cross-reactivity with rhIGF-I (< 0.01%) and insulin (< 0.01%). Compatibility of pairs of MAbs was tested in two-site IRMAs using three radioiodinated MAbs… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-8 of 8 extracted citations

Similar Papers

Loading similar papers…