Measurement of hydroxylysine glycosides in urine and its application to spinal cord injury.

@article{Rodriguez1984MeasurementOH,
  title={Measurement of hydroxylysine glycosides in urine and its application to spinal cord injury.},
  author={Gladys P Rodriguez and Jacqueline L Claus-Walker},
  journal={Journal of chromatography},
  year={1984},
  volume={308},
  pages={
          65-73
        }
}
A new technique to evaluate the degradation of skin or bone collagen by measuring glucosylgalactosyl hydroxylysine and galactosyl hydroxylysine is presented. The method utilizes an automated amino acid analyzer. Eluents used are lithium buffers, and the color reagent is ninhydrin. Both glycosides elute in 3.5 h. Samples require minimum preparation. Urinary concentrations of both glycosides in ten patients with cervical spinal cord injuries of less than six months duration were higher than in… CONTINUE READING