Measurement of human growth hormone in urine: development and validation of a sensitive and specific assay.

@article{Hourd1989MeasurementOH,
  title={Measurement of human growth hormone in urine: development and validation of a sensitive and specific assay.},
  author={P Hourd and Ray Edwards},
  journal={The Journal of endocrinology},
  year={1989},
  volume={121 1},
  pages={167-75}
}
A specific solid-phase immunoradiometric assay (IRMA), optimized for maximum sensitivity, has been developed for measurement of human GH (hGH) in urine. The sensitivity varied with sample size, giving a range of 0.001 to 0.003 mU/l for a sample volume of 2 ml. Recovery and dilution experiments, together with chromatography of urine samples, indicate that the method is specific for hGH. Added exogenous hGH was measured with a mean recovery of 101 +/- 10% (S.D.) for 1 ml samples and 87 +/- 8% for… CONTINUE READING