Measurement of hippocampal levels of cellular second messengers following in situ freezing.

@article{Tzigaret1993MeasurementOH,
  title={Measurement of hippocampal levels of cellular second messengers following in situ freezing.},
  author={C M Tzigaret and T K Mcintosh and K Okiyama and Walt L. Jenkins and Mani Ram Prasad},
  journal={Journal of neurochemistry},
  year={1993},
  volume={60 3},
  pages={827-34}
}
The in situ freezing technique has been widely used to fix labile metabolites and cellular second messengers in cerebral cortex. In this study, we isolated specific brain regions at 0 degree C from coronal sections of frozen heads following in situ brain freezing and measured regional concentrations of labile metabolites and cellular messengers. These levels in the cortex were compared with those in cortical punches obtained at freezing temperature (less than -40 degrees C) from the same in… CONTINUE READING