Measurement of fluorescence changes of NAD(P)H and of fluorescent flavoproteins in saponin-skinned human skeletal muscle fibers.

@article{Kunz1994MeasurementOF,
  title={Measurement of fluorescence changes of NAD(P)H and of fluorescent flavoproteins in saponin-skinned human skeletal muscle fibers.},
  author={Wolfram S Kunz and A. V. Kuznetsov and Kirstin Winkler and Frank Norbert Gellerich and S Neuhof and Hans Wolfram Neumann},
  journal={Analytical biochemistry},
  year={1994},
  volume={216 2},
  pages={322-7}
}
Saponin-skinned human muscle fibers from M. vastus lateralis were immobilized in a quartz capillary to detect the fluorescence changes of NAD(P)H and of fluorescent flavoproteins. To get sufficient intense fluorescence signals from a small amount of muscle tissue the NAD(P)H fluorescence was excited by means of an HeCd laser at 325 nm and the flavoprotein fluorescence by an argon-ion laser at 454 nm or by the second wavelength of a HeCd laser at 442 nm. Using this experimental setup the… CONTINUE READING
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