Measurement of flow rate in rat proximal tubules with a nonobstructing optical method.

@article{Chou1987MeasurementOF,
  title={Measurement of flow rate in rat proximal tubules with a nonobstructing optical method.},
  author={C L Chou and Donald J. Marsh},
  journal={The American journal of physiology},
  year={1987},
  volume={253 2 Pt 2},
  pages={F366-71}
}
A method was developed for measuring volume flow rate in free-flowing proximal tubules. Multiple injections of a solution containing fluorescein isothiocyanate-dextran (mol wt 20,000) were made into the tubular fluid stream while fluorescence was detected by videomicroscopy. Progression of the dye bolus between two points along the tubule was measured to determine fluid velocity. Tubule diameter was measured by video image shearing and flow rate was calculated from the product of velocity and… CONTINUE READING

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