Measurement of detergent concentration using 2,6-dimethylphenol in membrane-protein crystallization.

@article{Prince2012MeasurementOD,
  title={Measurement of detergent concentration using 2,6-dimethylphenol in membrane-protein crystallization.},
  author={Chelsy C. Prince and Zongchao Jia},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={2012},
  volume={68 Pt 12},
  pages={1694-6}
}
Methods have previously been developed to measure detergent concentration in membrane-protein samples, but most have significant limitations, such as requiring specialized equipment or consuming a significant amount of precious sample. This work explores the use of 2,6-dimethylphenol in a phenol-sulfuric acid assay to accurately measure the concentration of… CONTINUE READING