Measurement of cytosolic free magnesium ion concentration by 19F NMR.

  title={Measurement of cytosolic free magnesium ion concentration by 19F NMR.},
  author={L. A. Linden Levy and Elizabeth Murphy and B. Syam Prasad Raju and Robert E. London},
  volume={27 11},
Fluorinated derivatives of the chelator o-aminophenol-N,N,O-triacetic acid (APTRA) have been developed, synthesized, and analyzed for use as 19F NMR indicators of free cytosolic magnesium concentration. Magnesium dissociation constants for the 4-fluoro, 5-fluoro, and 4-methyl-5-fluoro species were determined to be 3.1, 0.9, and 0.6 mM, respectively, on the basis of UV absorption measurements at 37 degrees C in 115 mM KCl and 20 mM NaCl, pH 7.1, buffered with 4-(2-hydroxyethyl)-1… CONTINUE READING

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