Measurement of cytosolic-free Ca²⁺ in plant tissue.


A range of techniques have been used to measure the concentration of cytosolic-free Ca(2+) ([Ca(2+)](cyt)) in plant cells. Fluorescent Ca(2+)-sensitive indicators have been used extensively to measure plant [Ca(2+)](cyt) and a number of techniques are available for loading these into plant cells. Here we describe a method for measuring [Ca(2+)](cyt) in the guard cells of the model plant species Commelina communis by ratio photometry and imaging techniques using the ratiometric fluorescent Ca(2+)-sensitive indicator fura-2.

DOI: 10.1385/1-59259-949-4:289

Cite this paper

@article{McAinsh2005MeasurementOC, title={Measurement of cytosolic-free Ca²⁺ in plant tissue.}, author={Martin R. McAinsh and Carl K-Y Ng}, journal={Methods in molecular biology}, year={2005}, volume={312}, pages={289-302} }