Measurement of cytochrome P450 and NADPH–cytochrome P450 reductase

  title={Measurement of cytochrome P450 and NADPH–cytochrome P450 reductase},
  author={F Peter Guengerich and M. Virginia Mart{\'i}n and Christal D. Sohl and Qian Cheng},
  journal={Nature Protocols},
Cytochrome P450 (P450) enzymes are important in the metabolism of steroids, vitamins, carcinogens, drugs and other compounds. Two of the commonly used assays in this field are the measurements of total P450 and NADPH–P450 reductase in biological preparations. A detailed protocol is presented for the measurement of P450 by its spectral properties, along with a protocol for measuring NADPH–P450 reductase by its NADPH–cytochrome c reduction activity. Each assay can be completed in 5–10 min… CONTINUE READING