Measurement of cystine in granulocytes using liquid chromatography-tandem mass spectrometry.

@article{Chabli2007MeasurementOC,
  title={Measurement of cystine in granulocytes using liquid chromatography-tandem mass spectrometry.},
  author={Allel Chabli and Jo{\"e}lle Aupetit and M Raehm and Daniel Ricquier and Bernadette Chadefaux-Vekemans},
  journal={Clinical biochemistry},
  year={2007},
  volume={40 9-10},
  pages={692-8}
}
BACKGROUND Cystinosis is a rare autosomal recessive disorder characterized by an accumulation of intralysosomal cystine due to a defect in cystine transport across the lysosomal membrane. This disorder can be treated specifically using high doses of cysteamine. Accurate measurement of intracellular cystine content is necessary for the diagnosis and monitoring of treatment with cysteamine. Here we describe a new method to measure intracellular cystine. It relies on a liquid chromatography-tandem… CONTINUE READING