Measurement of circulating 25-hydroxyvitamin D: A historical review

  title={Measurement of circulating 25-hydroxyvitamin D: A historical review},
  author={Caroline Le Goff and Etienne Cavalier and J.-C. Souberbielle and A. Gonz{\'a}lez-Antu{\~n}a and Edgard E. Delvin},
  booktitle={Practical laboratory medicine},
The constantly increasing requests for the measurement of serum 25-hydroxyvitamin D over the last years has led reagent manufacturers to market different automated and semi-automated methods, that being unfortunately not fully harmonized, yield different results. Liquid chromatography coupled to tandem mass spectrometry (LC/MS2) has more recently been introduced. This approach allows the distinction between the two forms of 25-hydroxyvitamin D and to measure other metabolites. This approach… CONTINUE READING

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