Measurement of antigen specific lymphocyte proliferation using 5-bromo-deoxyuridine incorporation. An easy and low cost alternative to radioactive thymidine incorporation.

@article{Huong1991MeasurementOA,
  title={Measurement of antigen specific lymphocyte proliferation using 5-bromo-deoxyuridine incorporation. An easy and low cost alternative to radioactive thymidine incorporation.},
  author={Phan Le Thanh Huong and A. J. Kolk and Teunis A. Eggelte and C. P. H. J. Verstijnen and Henk Gilis and Jan T Hendriks},
  journal={Journal of immunological methods},
  year={1991},
  volume={140 2},
  pages={243-8}
}
The classical in vitro assay for the determination of cell mediated immune responses is the lymphocyte transformation test (LTT) in which cell proliferation is measured by incorporation of radioactive labeled thymidine (3H-TdR). The LTT assay using 3H-TdR is less suited for modestly equipped laboratories as it is costly, laborious and involves the need to handle radioactive isotopes and specialized equipment. Here we describe an improved alternative LTT method which is capable of detecting… CONTINUE READING

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