Measurement of anti-glomerular-basement-membrane antibodies by micro ELISA using insoluble antigens.

An enzyme immunoassay, using finely ground rabbit glomerular basement membrane (GBM) as an antigen, was able to detect sheep anti-rabbit GBM antibodies at serum dilutions of 1:32 000. The particulate GBM bound firmly to plastic micro ELISA plates without the aid of a linking agent, and the antigen-coated plates remained stable for several months when stored… (More)