Measurement of amide proton exchange rates and NOEs with water in 13C/15N-enriched calcineurin B.

@article{Grzesiek1993MeasurementOA,
  title={Measurement of amide proton exchange rates and NOEs with water in 13C/15N-enriched calcineurin B.},
  author={Stephan Grzesiek and Allison Bax},
  journal={Journal of biomolecular NMR},
  year={1993},
  volume={3 6},
  pages={
          627-38
        }
}
A rapid and sensitive 2D approach is presented for measuring amide proton exchange rates and the NOE interaction between amide protons and water. The approach is applicable to uniformly 13C/15N-enriched proteins and can measure magnetization exchange rates in the 0.02 to > 20 s-1 range. The experiments rely on selective excitation of the water resonance, coupled with purging of underlying H alpha resonances, followed by NOESY- or ROESY-type transfer to amide protons, which are dispersed by the… CONTINUE READING
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