Measurement of a follicle-stimulating hormone-responsive protein of Sertoli cell origin using an enzyme-linked immunoblot assay.

@article{Lee1986MeasurementOA,
  title={Measurement of a follicle-stimulating hormone-responsive protein of Sertoli cell origin using an enzyme-linked immunoblot assay.},
  author={W. M. Peter Lee and C. Yan Cheng and Caroline Bardin and Glen L Gunsalus and Neal A. Musto},
  journal={Endocrinology},
  year={1986},
  volume={119 5},
  pages={1914-21}
}
Many proteins secreted by Sertoli cell-enriched cultures are maximally stimulated by a combination of FSH and testosterone. Since very few are stimulated primarily by FSH, we thought it pertinent to identify such proteins. Sertoli cell-enriched cultures were prepared from testes of 20-day-old rats and grown in serum-free medium containing insulin, transferrin, and epidermal growth factor and in such medium supplemented with FSH, testosterone, or FSH plus testosterone. Media were fractionated… CONTINUE READING