Measurement of T and B cell turnover with bromodeoxyuridine.

  title={Measurement of T and B cell turnover with bromodeoxyuridine.},
  author={David F. Tough and Jonathan Sprent and Geoffrey L. Stephens},
  journal={Current protocols in immunology},
  volume={Chapter 4},
  pages={Unit 4.7}
This unit describes two methods used to determine the rate of turnover of T and B cells in vivo. In each method, this is accomplished by administering 5-bromo-2'-deoxyuridine (BrdU), a thymidine analog, and measuring the rate at which cells become labeled with this DNA precursor during the S phase of the cell cycle. Cells that contain BrdU are then detected… CONTINUE READING