Measurement of Serum Vitamin B12 Level Using Radioisotope Dilution and Coated Charcoal.

@article{Lau1965MeasurementOS,
  title={Measurement of Serum Vitamin B12 Level Using Radioisotope Dilution and Coated Charcoal.},
  author={Kam Shing Lau and Charles Gottlieb and L R Wasserman and Victor D Herbert},
  journal={Blood},
  year={1965},
  volume={26},
  pages={202-14}
}
I N PREVIOUS REPORTS’3 it was shown that uncoated charcoal adsorbs both free vitamin B12 and B12 bound to proteins, but charcoal precoated by mixing with saturating quantities of albumin adsorbs only free B12. This finding has been confirmed.4 The charcoal particles may be considered as solid microsponges and the albumin coat as a molecular sieve surrounding each sponge. Small molecules such as B12 pass through the coat and continue to be adsorbed, but large molecules such as proteins are… CONTINUE READING

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