Measurement of Placental Alpha-Microglobulin-1 in Cervicovaginal Discharge to Diagnose Rupture of Membranes

  title={Measurement of Placental Alpha-Microglobulin-1 in Cervicovaginal Discharge to Diagnose Rupture of Membranes},
  author={Si Eun Lee and Joong-Shin Park and Errol R. Norwitz and Kunwoo Kim and Hyun Soo Park and Jong Kwan Jun},
  journal={Obstetrics \& Gynecology},
OBJECTIVE: To compare the accuracy of an immunoassay to measure levels of placental alpha-microglobulin-1 in cervicovaginal secretions with that of conventional clinical assessment for the diagnosis of rupture of membranes. METHODS: A prospective observational study was performed in consecutive patients with signs or symptoms of rupture of membranes at Seoul National University Hospital from March 2005 to February 2006. Initial evaluation included both the standard clinical evaluation for… 

Placental α-microglobulin-1 to detect uncertain rupture of membranes in a European cohort of pregnancies

AT was more sensitive compared to clinical assessment, independent of the examiners experience and gestational age, and seems to be a cost-effective approach in the assessment of these patients with uncertain ROM.

Comparison of placental alpha microglobulin-1 in vaginal fluid with intra-amniotic injection of indigo carmine for the diagnosis of rupture of membranes

The PAMG-1 immunoassay in vaginal fluid yielded results that were comparable to those of the instillation of indigo carmine into the amniotic cavity; therefore, it is proposed that PAMg-1 is a sensitive and specific test to assess ROM in patients with an equivocal diagnosis based on simple tests.


Placental alpha-microglobulin-1 immunoassay is a rapid and accurate method for confirming the diagnosis of rupture of membrane and was superior to conventional standard diagnostic methods.

Diagnostic performance of placental alpha-microglobulin-1 test in women with prolonged pre-labour rupture of membranes

  • G. ElejeE. Ezugwu C. Ezeama
  • Medicine
    The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
  • 2016
This study in women with prolonged PROM, confirms that PAMG-1 test has high diagnostic accuracy irrespective of the duration of PROM before clinical evaluation.

Placental Alpha Microglobulin-1 Detection in Cervico-vaginal Secretions in the Diagnosis of Preterm Premature Rupture of The Membranes

The value of detection of placental alpha macroglobulin-1 in cervicovaginal discharge as a diagnostic test for Premature Rupture of Membranes (PROM) compared with traditional methods was evaluated.

Diagnosing rupture of membranes using combination monoclonal/polyclonal immunologic protein detection.

This combination monoclonal and polyclonal immunoassay test that detects PP12 and AFP has an efficacy comparable to conventional testing and better than the individual components of conventional testing (ferning, nitrazine).

Amnisure: A point of care diagnostic for preterm, term prelabor rupture of membranes

The aim was to determine efficacy of AmniSure rapid immunoassay placental alpha-microglobulin-1 test for accurate diagnosis of true ROM in women with watery discharge after 28 weeks gestation, compare with conventional methods.

Fetal fibronectin (Quick Check fFN test) versus placental alpha microglobulin-1 (AmniSure test) for detection of premature rupture of fetal membranes

fFN bedside test is better than nitrazine and ferning tests in detection of PROM, but is not suited for identification of clinically difficult cases with suspectedPROM, because, it is influenceable and can give false-positive results in preterm labor, women >34 gestational weeks and after any vaginal manipulation without PROM.

Novel markers for the diagnosis of premature rupture of membrane in pregnancy: urea, creatinine and prolactin of vaginal fluid

  • R. GargA. Nigam
  • Medicine
    International Journal of Clinical Obstetrics and Gynaecology
  • 2019
Prolactin has more diagnostic value than urea and creatinine in detecting PROM, and can be used in suspected cases, and was the best in predicting PROM.



AmniSure placental alpha microglobulin-1 rapid immunoassay versus standard diagnostic methods for detection of rupture of membranes.

The purpose of this study was to compare the AmniSure rapid immunoassay with standard methods for diagnosing rupture of fetal membranes and found it to be highly accurate in diagnosing fetal membrane rupture.

Value of the fern test to confirm or reject the diagnosis of ruptured membranes is modest in nonlaboring women presenting with nonspecific vaginal fluid loss.

It is concluded that the fern test should be granted supportive rather than conclusive value in diagnosing ruptured membranes in nonlaboring women presenting with nonspecific vaginal fluid loss.

Prolonged rupture of membranes in the term newborn.

A conservative clinical approach utilizing blood cultures and CBC evaluations in the management of PROM is warranted, asPROM is associated with significantly increased incidence of positive blood cultures.

Preterm Premature Rupture of Membranes, Intrauterine Infection, and Oligohydramnios: Risk Factors for Placental Abruption

Women presenting with preterm PROM are at increased risk of developing abruption, with the risk being higher either in the presence of intrauterine infections or oligohydramnios.

Ferning of Amniotic Fluid Contaminated With Blood

The presence of blood may alter the morphology of the fern, but does not act as a contaminant that would affect the accuracy of the test.

Clinical significance of intra-amniotic inflammation in patients with preterm premature rupture of membranes.

The relationship between oligohydramnios and the onset of preterm labor in preterm premature rupture of membranes.

Patients with preterm premature rupture of membranes and an amniotic fluid index of < or = 5 cm are at increased risk for a shorter interval to delivery.

Significance of detecting insulin‐like growth factor binding protein‐1 in cervicovaginal secretions: comparison with nitrazine test and amniotic fluid volume assessment

The accuracy of detecting insulin‐like growth factor binding protein‐1 (IGFBP‐1) in vaginal fluid by a rapid dipstick method in comparison with the nitrazine test and amniotic fluid index (AFI) and the predictivity of these methods on the latency of pregnancy in patients with suspected PROM is studied.