Measurement of GLUT mRNA in liver of fetal and neonatal rats using a novel method of quantitative polymerase chain reaction.

@article{Lane1996MeasurementOG,
  title={Measurement of GLUT mRNA in liver of fetal and neonatal rats using a novel method of quantitative polymerase chain reaction.},
  author={Robert Hooijberg Nancy Lane and Annette S. Flozak and Rebecca A. Simmons},
  journal={Biochemical and molecular medicine},
  year={1996},
  volume={59 2},
  pages={192-9}
}
Transfer of glucose into the hepatocyte is mediated by glucose transporters (GLUTs). GLUT mRNA levels are usually measured by Northern blot analysis. Reverse transcription-polymerase chain reaction (RT-PCR) is often used to measure RNA abundance. However, this method is only semiquantitative and has no internal control during first-strand synthesis. We designed a method of coreverse transcription and PCR amplification using bovine rhodopsin as an internal control for both cDNA synthesis and… CONTINUE READING

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