Measurement of DNA base damage in cells exposed to low doses of gamma-radiation: comparison between the HPLC-EC and comet assays.

@article{Pouget1999MeasurementOD,
  title={Measurement of DNA base damage in cells exposed to low doses of gamma-radiation: comparison between the HPLC-EC and comet assays.},
  author={Jean-Pierre Pouget and J L Ravanat and Thierry Douki and Marie Jeanne Richard and Jean Cadet},
  journal={International journal of radiation biology},
  year={1999},
  volume={75 1},
  pages={51-8}
}
PURPOSE Two different methods aimed at measuring DNA damage were compared. Monocytes were exposed to 60Co gamma-rays and the level of DNA damage was determined using either the HPLC-EC or comet assay. MATERIALS AND METHODS The alkaline comet assay was used in association with the Fpg and Endo III DNA glycosylases to estimate the amount of modified bases together with strand breaks and alkali-labile sites. The HPLC-EC analysis was performed to measure 8-oxo-7,8-dihydro-2'-deoxyguanosine (8… CONTINUE READING