Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry.

@article{Korth2019MeasurementOB,
  title={Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry.},
  author={J. Korth and Helene Sertznig and S. Moyrer and A. Doevelaar and T. Westhoff and N. Babel and O. Witzke and A. Kribben and U. Dittmer and M. Widera},
  journal={Journal of visualized experiments : JoVE},
  year={2019},
  volume={149}
}
Polyomaviruses, like the BK-polyomavirus (BKPyV), can cause severe pathologies in immunocompromised patients. However, since highly effective antivirals are currently not available, methods measuring the impact of potential antiviral agents are required. Here, a dual fluorescence reporter that allows the analysis of the BKPyV non-coding control-region (NCCR) driven early and late promoter activity was constructed to quantify the impact of potential antiviral drugs on viral gene expression via… Expand