Measurement by reversed-phase high-performance liquid chromatography of malondialdehyde in normal human urine following derivatisation with 2,4-dinitrophenylhydrazine.

@article{Korchazhkina2003MeasurementBR,
  title={Measurement by reversed-phase high-performance liquid chromatography of malondialdehyde in normal human urine following derivatisation with 2,4-dinitrophenylhydrazine.},
  author={Olga V Korchazhkina and Christopher Exley and Stephen Andrew Spencer},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2003},
  volume={794 2},
  pages={
          353-62
        }
}
  • Olga V Korchazhkina, Christopher Exley, Stephen Andrew Spencer
  • Published in
    Journal of chromatography. B…
    2003
  • Chemistry, Medicine
  • A selective and sensitive method based on derivatisation with 2,4-dinitrophenylhydrazine (DNPH) and consecutive HPLC gradient separation is described for the determination of malondialdehyde (MDA) in urine. Preparation of urine samples involved a one-step derivatisation/extraction procedure. Separation was achieved using a Waters SymmetryC(18) column (3.9 x 150 mm) and linear gradient of acetonitrile in water (from 30% to 70% in 30 min). The overall detection limit of the method was 56 nM of… CONTINUE READING

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