Measurement and pharmacokinetics of unbound 20(S)-camptothecin in rat blood and brain by microdialysis coupled to microbore liquid chromatography with fluorescence detection.

@article{Tsai2000MeasurementAP,
  title={Measurement and pharmacokinetics of unbound 20(S)-camptothecin in rat blood and brain by microdialysis coupled to microbore liquid chromatography with fluorescence detection.},
  author={Tung-Hu Tsai and Yunjia Chen and Cheng Jen Chou and Chieh Fu Chen},
  journal={Journal of chromatography. A},
  year={2000},
  volume={870 1-2},
  pages={221-6}
}
To characterize the pharmacokinetics of protein-free camptothecin in blood and brain we implanted microdialysis probes into the jugular vein and striatum of rats for unbound drug sampling and determination. Camptothecin (2 or 5 mg/kg, i.v., n=6) was then administered from the femoral vein, and microdialysates were collected from blood and brain of both sites and assayed by a validated microbore scale high-performance liquid chromatographic method. The mobile phase consisted of methanol-100 mM… CONTINUE READING