Measurement and clinical monitoring of human lymphocyte clonality by massively parallel VDJ pyrosequencing.

@article{Boyd2009MeasurementAC,
  title={Measurement and clinical monitoring of human lymphocyte clonality by massively parallel VDJ pyrosequencing.},
  author={Scott D Boyd and Eleanor L. Marshall and Jason Merker and Jay Mahesh Maniar and Lyndon N. Zhang and Bita Sahaf and Carol Jones and Birgitte B. Simen and Bozena Hanczaruk and Khoa Duy Nguyen and Kari Christine Nadeau and Michael Egholm and D. Mikl{\'o}s and James L Zehnder and Andrew Z Fire},
  journal={Science translational medicine},
  year={2009},
  volume={1 12},
  pages={12ra23}
}
The complex repertoire of immune receptors generated by B and T cells enables recognition of diverse threats to the host organism. In this work, we show that massively parallel DNA sequencing of rearranged immune receptor loci can provide direct detection and tracking of immune diversity and expanded clonal lymphocyte populations in physiological and pathological contexts. DNA was isolated from blood and tissue samples, a series of redundant primers was used to amplify diverse DNA… CONTINUE READING
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