MeCAT labeling for absolute quantification of intact proteins using label-specific isotope dilution ICP-MS

@inproceedings{EstebanFernandez2012MeCATLF,
  title={MeCAT labeling for absolute quantification of intact proteins using label-specific isotope dilution ICP-MS},
  author={D. Esteban-Fernandez and Frank S Bierkandt and Michael W. Linscheid},
  year={2012}
}
Sensitive, accurate and fast absolute quantification of intact proteins is reported using metal coded affinity tags (MeCATs) combined with gel electrophoresis (GE) separation, inductively coupled plasma mass spectrometry (ICP-MS) detection and label-specific isotope dilution analysis quantification. Two different approaches were investigated concerning the introduction of the sample into ICP-MS, mineralization of the gel and direct laser ablation (LA) of the spot. Three major advantages are… CONTINUE READING