MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli.

@article{Zhang2003MazFCC,
  title={MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli.},
  author={Y Zhang and Junjie Zhang and Klaus P. Hoeflich and Mitsuhiko Ikura and G Qing and M. Inouye},
  journal={Molecular cell},
  year={2003},
  volume={12 4},
  pages={913-23}
}
Escherichia coli contains operons called "addiction modules," encoding toxin and antitoxin, which are responsible for growth arrest and cell death. Here, we demonstrate that MazF toxin encoded by "mazEF addiction module" is a sequence-specific (ACA) endoribonuclease functional only for single-stranded RNA. MazF works as a ribonuclease independent of ribosomes, and is, therefore, functionally distinct from RelE, another E. coli toxin, which assists mRNA cleavage at the A site on ribosomes. Upon… CONTINUE READING
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Thus , MazF functions as a toxic endoribonuclease to interfere with the function of cellular mRNAs by cleaving them at specific sequences leading to rapid cell growth arrest and cell death .
Thus , MazF functions as a toxic endoribonuclease to interfere with the function of cellular mRNAs by cleaving them at specific sequences leading to rapid cell growth arrest and cell death .
The role of such endoribonucleases may have broad implication in cell physiology under various growth conditions .
The role of such endoribonucleases may have broad implication in cell physiology under various growth conditions .
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