Maturation of parvovirus LuIII in a subcellular system. II. Isolation and characterization of nucleoprotein intermediates.

@article{Muller1983MaturationOP,
  title={Maturation of parvovirus LuIII in a subcellular system. II. Isolation and characterization of nucleoprotein intermediates.},
  author={David E. Muller and Gunter Siegl},
  journal={The Journal of general virology},
  year={1983},
  volume={64 Pt 5},
  pages={1055-67}
}
Nucleoprotein (NP) intermediates in the assembly pathway of parvovirus LuIII were isolated. These structures consist of replicating viral DNA and of preformed viral capsids into which progeny viral DNA is encapsidated concomitant with synthesis. The NP complexes sediment at between 70S and 100S. They are unstable in CsCl and dissociate in the presence of 1 M-NaCl. After fixation with glutaraldehyde, however, they accumulate at a density of 1.37 g/ml in CsCl gradients. Electron microscopy of… CONTINUE READING

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