Maturation of Pseudomonas aeruginosa elastase. Formation of the disulfide bonds.

@article{Braun2001MaturationOP,
  title={Maturation of Pseudomonas aeruginosa elastase. Formation of the disulfide bonds.},
  author={Paula Braun and C. Ockhuijsen and Elaine F. Eppens and M. Koster and Wilbert Bitter and Jan P Tommassen},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 28},
  pages={26030-5}
}
Elastase of Pseudomonas aeruginosa is synthesized as a preproenzyme. After propeptide-mediated folding in the periplasm, the proenzyme is autoproteolytically processed, prior to translocation of both the mature enzyme and the propeptide across the outer membrane. The formation of the two disulfide bonds present in the mature enzyme was examined by studying the expression of the wild-type enzyme and of alanine for cysteine mutant derivatives in the authentic host and in dsb mutants of… CONTINUE READING

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