Maturation of [NiFe]-hydrogenases in Escherichia coli: the HypC cycle.

  title={Maturation of [NiFe]-hydrogenases in Escherichia coli: the HypC cycle.},
  author={Melanie Blokesch and August Boeck},
  journal={Journal of molecular biology},
  volume={324 2},
Carbamoyl phosphate (CP) has been implicated as an educt for the synthesis of the CO and CN ligands of the metal centre of [NiFe]-hydrogenases in Escherichia coli, since CP synthetase mutants (carAB) are unable to generate active hydrogenases due to a block in enzyme maturation. Citrulline, when added to the growth medium in high concentrations, compensated for the phenotype of the mutants. It is now shown that overexpression of the argI gene lowered the effective concentration of citrulline… CONTINUE READING