Maturation and germination of walnut somatic embryos

  title={Maturation and germination of walnut somatic embryos},
  author={Ming-De Deng and Daniel Cornu},
  journal={Plant Cell, Tissue and Organ Culture},
Walnut somatic embryos were multiplied by repetitive embryogenesis on a solid basal DKW medium at 25°C in the dark. When the embryos were isolated at early cotyledonary stage (1–2 mm long) from the primary embryos and cultured on the medium for 3 weeks, they developed into mature embryos showing white, enlarged cotyledons and shoot and root apex. After transfer to light on solid germination medium, however, few mature embryos (0–5%) germinated. Germination percentage increased to about 10% when… CONTINUE READING
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