Maturation and fertilization of equine oocytes.

  title={Maturation and fertilization of equine oocytes.},
  author={Edward L. Squires},
  journal={The Veterinary clinics of North America. Equine practice},
  volume={12 1},
  • Edward L. Squires
  • Published 1996 in
    The Veterinary clinics of North America. Equine…
Equine oocytes obtained either by transvaginal ultrasound-guided follicular aspiration or from slaughterhouse ovaries can be matured in vitro. This generally requires culture in TCM-199 containing serum and hormones for 30 to 36 hours. With this protocol, approximately 50% to 60% of the oocytes are at metaphase-II at the end of the culture period. At least some of these oocytes appear viable based on production of fertilized eggs either through in vitro fertilization or fertilization in vivo of… CONTINUE READING