Matrix polysaccharide precursors in Arabidopsis cell walls are synthesized by alternate pathways with organ-specific expression patterns.

@article{Seitz2000MatrixPP,
  title={Matrix polysaccharide precursors in Arabidopsis cell walls are synthesized by alternate pathways with organ-specific expression patterns.},
  author={Barbara Seitz and C. Klos and Martin Wurm and Raimund Tenhaken},
  journal={The Plant journal : for cell and molecular biology},
  year={2000},
  volume={21 6},
  pages={537-46}
}
The expression pattern of the single-copy gene UDP-glucose dehydrogenase (Ugd) was analysed in transgenic Arabidopsis plants by promoter:GUS and GFP fusions, Western blots, activity assays and histochemical activity staining. The enzyme oxidizes UDP-glucose to UDP-glucuronic acid and thus directs carbohydrates irreversibly into a cell wall-specific pool of nucleotide sugars. UDP-glucuronic acid is the central intermediate in the interconversion pathway to other nucleotide sugars, including the… CONTINUE READING