Matrix metalloproteinase (MMP)-2 and MMP-9 activities in human seminal plasma.

  title={Matrix metalloproteinase (MMP)-2 and MMP-9 activities in human seminal plasma.},
  author={Ken-ichi Shimokawa Ki and Masatoki Katayama and Yoshifumi Matsuda and Hidenobu Takahashi and Izumi Hara and Hirohisa Sato and Satoru Kaneko},
  journal={Molecular human reproduction},
  volume={8 1},
We report on the existence of two kinds of matrix metalloproteinases (MMPs), MMP-2 and MMP-9, in human seminal plasma. Partial purification of the proteinases was achieved by two steps, consisting of chromatography on a gel-filtration column and then on a gelatin affinity column. Proteinase activities in the chromatography extracts were shown to hydrolyse a fluorescent substrate specific to MMPs (Dnp-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2). The proteinases were detected using gelatin-zymography, but… CONTINUE READING


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