Matrix-free UV-laser desorption/ionization (LDI) mass spectrometric imaging at the single-cell level: distribution of secondary metabolites of Arabidopsis thaliana and Hypericum species.

@article{Hlscher2009MatrixfreeUD,
  title={Matrix-free UV-laser desorption/ionization (LDI) mass spectrometric imaging at the single-cell level: distribution of secondary metabolites of Arabidopsis thaliana and Hypericum species.},
  author={Dirk H{\"o}lscher and Rohit Shroff and Katrin rer. nat. Knop and Michael Gottschaldt and Anna Crecelius and Bernd Schneider and David G. Heckel and Ulrich S. Schubert and Ale{\vs} Svato{\vs}},
  journal={The Plant journal : for cell and molecular biology},
  year={2009},
  volume={60 5},
  pages={
          907-18
        }
}
The present paper describes matrix-free laser desorption/ionisation mass spectrometric imaging (LDI-MSI) of highly localized UV-absorbing secondary metabolites in plant tissues at single-cell resolution. The scope and limitations of the method are discussed with regard to plants of the genus Hypericum. Naphthodianthrones such as hypericin and pseudohypericin are traceable in dark glands on Hypericum leaves, placenta, stamens and styli; biflavonoids are also traceable in the pollen of this… CONTINUE READING
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