Matrix elasticity, replicative senescence and DNA methylation patterns of mesenchymal stem cells.

@article{Schellenberg2014MatrixER,
  title={Matrix elasticity, replicative senescence and DNA methylation patterns of mesenchymal stem cells.},
  author={Anne Schellenberg and Sylvia Joussen and Kristin Moser and Nico Hampe and Nils Hersch and Hatim Hemeda and Jan Schnitker and Bernd Denecke and Qiong Lin and Norbert Pallua and Martin Zenke and Rudolf Merkel and Bernd Hoffmann and Wolfgang Wagner},
  journal={Biomaterials},
  year={2014},
  volume={35 24},
  pages={6351-8}
}
Matrix elasticity guides differentiation of mesenchymal stem cells (MSCs) but it is unclear if these effects are only transient - while the cells reside on the substrate - or if they reflect persistent lineage commitment. In this study, MSCs were continuously culture-expanded in parallel either on tissue culture plastic (TCP) or on polydimethylsiloxane (PDMS) gels of different elasticity to compare impact on replicative senescence, in vitro differentiation, gene expression, and DNA methylation… CONTINUE READING