Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.

@article{Prodhom2010MatrixassistedLD,
  title={Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.},
  author={Guy Prod'hom and Alain Bizzini and Christian Durussel and Jacques Bille and Gilbert Greub},
  journal={Journal of clinical microbiology},
  year={2010},
  volume={48 4},
  pages={1481-3}
}
An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising. 
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