Matrix-assisted in vitro refolding of Pseudomonas aeruginosa class II polyhydroxyalkanoate synthase from inclusion bodies produced in recombinant Escherichia coli.

@article{Rehm2001MatrixassistedIV,
  title={Matrix-assisted in vitro refolding of Pseudomonas aeruginosa class II polyhydroxyalkanoate synthase from inclusion bodies produced in recombinant Escherichia coli.},
  author={Bernd H A Rehm and Qing-sheng Qi and Br Bernd Beermann and H. J. Hinz and Alexander Steinb{\"u}chel},
  journal={The Biochemical journal},
  year={2001},
  volume={358 Pt 1},
  pages={263-8}
}
In order to facilitate the large-scale preparation of active class II polyhydroxyalkanoate (PHA) synthase, we constructed a vector pT7-7 derivative that contains a modified phaC1 gene encoding a PHA synthase from Pseudomonas aeruginosa possessing six N-terminally fused histidine residues. Overexpression of this phaC1 gene under control of the strong Ø10 promoter was achieved in Escherichia coli BL21(DE3). The fusion protein was deposited as inactive inclusion bodies in recombinant E. coli, and… CONTINUE READING
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