Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize

@inproceedings{Kelliher2016MaternalHA,
  title={Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize},
  author={Timothy Kelliher and D Barry Starr and Wenling Wang and Jamie L McCuiston and Heng Zhong and Michael L Nuccio and Barry G H Martin},
  booktitle={Front. Plant Sci.},
  year={2016}
}
Doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. To test if haploid inducer lines can be engineered into crops, CENH3 (-∕-) and CENH3:RNAi lines were complemented by AcGREEN-tailswap-CENH3 or AcGREEN-CENH3 transgenes. Haploid induction rates were determined following testcrosses to wild-type plants after independently controlling for inducer parent sex and transgene zygosity. CENH3 fusion proteins were… CONTINUE READING
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