Material stiffness influences the polarization state, function and migration mode of macrophages.

  title={Material stiffness influences the polarization state, function and migration mode of macrophages.},
  author={Rukmani Sridharan and Brenton L. Cavanagh and Andrew R. Cameron and Daniel J. Kelly and Fergal Joseph O'Brien},
  journal={Acta biomaterialia},

Compliant Substrates Enhance Macrophage Cytokine Release and NLRP3 Inflammasome Formation During Their Pro-Inflammatory Response

It is shown that substrate stiffness modulates the pro-inflammatory response of macrophages, that the NLRP3 inflammasome is one of the components affected by macrophage mechanosensing, and a role for actomyosin contractility in this mechanosensory response.

Control of Matrix Stiffness Using Methacrylate-Gelatin Hydrogels for a Macrophage-Mediated Inflammatory Response.

The data demonstrated that macrophage behavior and the further inflammatory response are mechanically regulated by hydrogel stiffness, which can provide new insights into the future design and application of novel hydrogels-based biomaterials.

Macrophage phenotype and function are dependent upon the composition and biomechanics of the local cardiac tissue microenvironment

The present study sought to better elucidate the role that biochemical and biomechanical alterations in cardiac tissue have in the altered phenotype and functionality of cardiac resident macrophages observed with increasing age and suggests both biomechanicals and biochemical changes in the cardiovascular system play a role in promoting the age-related shift towards pro-inflammatory macrophage populations associated with cardiovascular disease development.

Matrix stiffness‐mediated effects on macrophages polarization and their LOXL2 expression

Increased matrix stiffness remarkably strengthened M2 macrophage polarization and promoted their LOXL2 expression in HCC tissues with COL1High/LOXHigh, further confirming the regulation roles of matrix stiffness in macrophages polarization and LO XL2 expression.

Advanced Strategies for Modulation of the Material–Macrophage Interface

The most relevant strategies that modulate biomaterial immune response are discussed, including mechanical properties, surface coatings, release of anti‐inflammatory molecules and cytokines, antibacterial features, origin and inner moieties of biomaterials, and cell crosstalk.

A 'Sandwich' Cell Culture Platform with NIR-Responsive Dynamic Stiffness to Modulate Macrophage Phenotypes

A novel “sandwich” cell culture platform with near-infrared (NIR) responsive dynamic stiffness was fabricated to polarize macrophage in situ for revealing the relationship between macrophages phenotype and substrate stiffness dynamically.

The Influence of Polysaccharides-Based Material on Macrophage Phenotypes.

This review focuses on recent advances in promoting wound healing and balancing macrophage phenotypes using polysaccharide-based substrates/coatings and new directions to address the limitations in the current understanding of Macrophage responses to poly Saccharides.

A "sandwich" cell culture platform with NIR-responsive dynamic stiffness to modulate macrophage phenotypes.

A novel "sandwich" cell culture platform with near-infrared (NIR) responsive dynamic stiffness was fabricated to polarize bone marrow-derived macrophages in situ for revealing the relationship between the macrophage phenotype and substrate stiffness dynamically.

Collagen Fibril Density Modulates Macrophage Activation and Cellular Functions during Tissue Repair

Through the investigation of macrophage-associated functions during tissue repair, it is demonstrated that M1LPS/IFNγ has the potential to enhance monocyte infiltration into tissue, while MIL-4/IL-13 supported fibroblast differentiation into myofibroblasts via transforming growth factor beta 1 in dependence of fibril density, suggesting a M2a-like phenotype.



Modulation of macrophage phenotype by cell shape

It is demonstrated here that elongation itself, without exogenous cytokines, leads to the expression of M2 phenotype markers and reduces the secretion of inflammatory cytokine, suggesting an important role for cell shape in regulating macrophage function.

Substrate Stiffness Regulates Proinflammatory Mediator Production through TLR4 Activity in Macrophages

Proinflammatory mediator production initiated by TLR4 is mechanically regulated in macrophages, suggesting that tissue stiffness affects macrophage activity and proinflammation on stiff substrates.

The effects of substrate stiffness on the in vitro activation of macrophages and in vivo host response to poly(ethylene glycol)-based hydrogels.

Hydrogels with lower stiffness led to reduced macrophage activation and a less severe and more typical FBR, and therefore are more suited for in vivo tissue engineering applications.

Sensitivity of alveolar macrophages to substrate mechanical and adhesive properties.

The results suggest that macrophages are able to probe their physical environment but the mechanosensitive mechanism behind appears quite different from tissue cells, since it occurs at no significant cell-scale prestress, shape changes through minimal actin remodeling and finally an AMs stiffness not affected by the loss in F-actin integrity.

Directing osteogenic and myogenic differentiation of MSCs: interplay of stiffness and adhesive ligand presentation.

The modulation of myogenic and osteogenic transcription factors by various ECM proteins demonstrates that substrate stiffness alone does not direct stem cell lineage specification, which has important implications in the development of tailored biomaterial systems that more closely mimic the microenvironment found in native tissues.

Macrophage plasticity and polarization: in vivo veritas.

The identification of mechanisms and molecules associated with macrophage plasticity and polarized activation provides a basis for Macrophage-centered diagnostic and therapeutic strategies.

Material surfaces affect the protein expression patterns of human macrophages: A proteomics approach.

It is demonstrated for the first time that a proteomics approach was able to detect protein expression profile changes in Monocyte-derived macrophages cultured on different material surfaces, forming the basis for utilizing further quantitative proteomics techniques that could assist in elucidation of the mechanisms involved in MDM-material interaction.