Mast cells promote fibroblast populated collagen lattice contraction through gap junction intercellular communication

  title={Mast cells promote fibroblast populated collagen lattice contraction through gap junction intercellular communication},
  author={Kurtis E. Moyer and Gregory C. Saggers and H. Paul Ehrlich},
  journal={Wound Repair and Regeneration},
The release of mast cell granules is commonly associated with inflammation and fibrosis. However, does direct communication between mast cells and fibroblasts through gap junction intercellular communication (GJIC) occur? Fibroblast populated collagen lattice (FPCL) cast with mast cells show enhanced lattice contraction. Do released granules or GJIC between mast cells and fibroblasts promote enhanced lattice contraction? Mast cells preloaded with a fluorescent dye that readily passes through… 

Rat Mast Cells Enhance Fibroblast Proliferation and Fibroblast-Populated Collagen Lattice Contraction through Gap Junctional Intercellular Communications

Heterocellular gap junctional intercellular communications with mast cells increase in fibro Blast proliferation and fibroblast PCL contraction, two hypertrophic scar fibroblasts activities.

Through Gap Junction Communications, Co-Cultured Mast Cells and Fibroblasts Generate Fibroblast Activities Allied with Hypertrophic Scarring

Eliminating the mast cell or its gap junctional intercellular communications with fibroblasts may be a possible approach in preventing hypertrophic scarring or reducing fibrotic conditions.

Rat mast cells communicate with fibroblasts via gap junction intercellular communications

It is proposed that GJIC between granulation tissue fibroblasts and MCs can modulate some aspects of wound repair and fibrosis.

MMP dependence of fibroblast contraction and collagen production induced by human mast cell activation in a three-dimensional collagen lattice.

It is demonstrated that activation of primary human mast cells with IgE receptor cross-linking, or activation of HMC-1 with C5a, enhanced contractility of human lung fibroblasts in a three-dimensional collagen lattice system.

Premature Collagen Fibril Formation, Fibroblast-Mast Cell Interactions and Mast Cell-Mediated Phagocytosis of Collagen in Keloids

The observation of early formation in keloid tissue of premature insoluble collagen fibrils supports previous studies that enzymes such as procollagen C-proteinase are important early therapeutic targets.

Role of Intercellular Interactions between Mast Cells and Gingival Fibroblasts in Mediating Inflammation

It is concluded that mast cells adhere to fibroblasts and promote IL-8 secretion, thereby enhancing neutrophil chemotaxis and possibly the perpetuation of the inflammatory response.

Intercellular interactions between mast cells and stromal fibroblasts obtained from canine cutaneous mast cell tumours

The establishment of primary cocultures of neoplastic mast cells from canine cutaneous MCTs and cancer-associated fibroblasts and the use of conditioned medium from co-cultured cells led to a significantly higher tumoral mast cell viability when in monoculture.

A Snapshot of Direct Cell-Cell Communications in Wound Healing and Scarring.

  • H. Ehrlich
  • Biology, Medicine
    Advances in wound care
  • 2013
GJIC between mast cells and fibroblasts during the remodeling phase of repair may explain how mast cells promote excessive scarring and the association of Cx with microtubules altering TGF-β signaling presents a new target for improving the quality of repair.

Cell-populated collagen lattice contraction model for the investigation of fibroblast collagen interactions.

In this chapter methods are described for manufacturing collagen lattices, which assess the three forces involved in compacting and/or organizing collagen fibrils into thicker collagen fibers.



Mast cells enhance contraction of three‐dimensional collagen lattices by fibroblasts by cell–cell interaction: role of stem cell factor/c‐kit

The results indicate that mast cells enhance fibroblast‐mediated contraction of collagen lattices via direct cell–cell contact, mediated in part by SCF/c‐kit interactions.

Co-Cultured Human Mast Cells Stimulate Fibroblast-Mediated Contraction of Collagen Gels

The hypothesis that the activity of mast cells may drive rearrangement of extracellular matrix and this and could subsequently lead to fibrosis and tissue dysfunction is supported.

Human mast cells activate fibroblasts: tryptase is a fibrogenic factor stimulating collagen messenger ribonucleic acid synthesis and fibroblast chemotaxis.

Analysis of type a1(I) procollagen mRNA synthesis by in situ hybridization revealed a substantial increase in mRNA levels in the presence of mast cells and especially following degranulation, induced by calcium ionophore A23187.

Mast cell and myofibroblast in wound healing.

Biochemical and functional characterization of intercellular adhesion and gap junctions in fibroblasts.

This donor-acceptor cell model allows large numbers of cells to form synchronous cell-to-cell contacts, thereby enabling the simultaneous functional and molecular studies of adherens junctions and gap junctions in human gingival fibroblasts.

Wound healing: the role of gap junctional communication in rat granulation tissue maturation.

Polarized light microscopy of Sirius red-stained specimens revealed more intense collagen fiber birefringence secondary to dense, parallel-organized collagen fiber bundles after LiCl treatment, suggesting that LiCl enhancement of GJIC between fibroblasts advances the maturation of granulation tissue.

Mast cells express connexins on their cytoplasmic membrane.

Expression of basic fibroblast growth factor and its receptor by fibroblast, macrophages and mast cells in hypertrophic scar.

An immunohistochemical study on bFGF and bF GF receptor in hypertrophic scar (HS) including keloid, in comparison with normal scar (non-HS) and normal skin found no obvious difference between non-HS and normal Skin, but Cellular source of the abnormal bFGI in HS may be both fibroblasts themselves and macrophages.

Junctional communication is induced in migrating capillary endothelial cells

Junctional communication may play an important role in wound repair, possibly by coordinating capillary endothelial cell migration, using an in vitro model of gap junction-mediated intercellular communication.